False-color image of the collagen-elastin structure by 2-photon tomography in vivo. The distribution of collagen is shown in red and that of elastin in green.

Determination of physiological skin parameters and of skin lesions

Determination of physiological skin parameters such as transepidermal water loss (TEWL), pH, Stratum corneum hydration, sebumetry, concentration of antioxidants, analysis of skin roughness and melanin distribution, in vivo characterization of the skin at a cellular level, characterization of wound healing and inflammatory processes, determination of collagen and elastin structures in the skin, analysis of the phase distribution (o/w or w/o) of formulations in the product and on the skin, in vivo analysis of the changes in the lipid structure of the skin during dermatological and cosmetic treatment, analysis of the efficacy of barrier creams and of barrier recovery, ethnic differences of skin parameters.

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Methods

  • pH-measurement
  • Corneometry
  • Sebumetry
  • Mexameter
  • Chromameter
  • In vivo laser scanning microscopy
  • Analysis of the skin surface structure (skin roughness)
  • Optical coherent tomography
  • Two-photon microscopy combined with FLIM and CARS (in vivo, ex vivo, in vitro)
  • TEWL
  • Confocal Raman microscopy combined with SERS (in vivo, ex vivo, in vitro)
  • Resonance Raman spectroscopy
Depiction of the Stratum corneum by a Laser scanning microscope, showing a regular organization of the corneocytes.
Skin barrier characterisation using in vivo laser scanning microscopy – healthy skin barrier. Photo: H. Richter/Charité.
Depiction of the Stratum corneum by a Laser scanning microscope, showing an irregular organization of the corneocytes.
Skin barrier characterisation using in vivo laser scanning microscopy – disturbed skin barrier. Photo: H. Richter/Charité.